A quick and large-scale density gradient subfractionation method for low density lipoproteins.
نویسندگان
چکیده
منابع مشابه
A quick and large-scale density gradient subfractionation method for low density lipoproteins.
A quick density gradient-banding subfractionation method has been developed for d < 1.063 g/ml lipoproteins. Up to 324 ml of plasma can be resolved into five distinct layers by a single ultracentrifugation. The separation was achieved with a discontinuous density gradient formed between plasma and a layer of NaCl solution of d 1.080 g/ml in an angle-head rotor during centrifugation at 45,000 rp...
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A simple method is described for precipitation of low density lipoproteins. The results correlate well with those from ultracentrifugation in combination with polyanion precipitation (135 +/- 35 vs 136 +/- 35, r = 0.984, n = 13) or quantitative lipoprotein electrophoresis (r = 0.9287, n = 6394). In addition to lipoprotein analysis, this method may be used for the extracorporeal elimination of ...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1982
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38170-0